Bone Marrow Aspiration Needle

ABSTRACT

Disclosed is an aspiration assembly including an outer cannulated needle having a tip, a threaded collar, and an inner aspiration needle. A material can be aspirated through at least the inner aspiration needle. The distance that the tip of the inner aspiration needle extends from the tip of the outer cannulated needle changes when the inner aspiration needle is rotated relative to the outer cannulated needle.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. patent application Ser. No.12/210,372 filed on Sep. 15, 2008. The entire disclosure of the aboveapplication is incorporated herein by reference.

FIELD

The present disclosure relates to methods and apparatuses for extractingbiological materials. In particular, the present disclosure relates tomethods and apparatuses for withdrawing bone marrow from bone.

BACKGROUND

The statements in this section merely provide background informationrelated to the present disclosure and may not constitute prior art.

Bone marrow aspiration is a procedure used to obtain the blood-formingportion (marrow) of the inner core of bone for examination in alaboratory or for transplantation. The procedure often includesinserting a needle into a bone that contains marrow and withdrawing themarrow. When harvesting large amounts of bone marrow, such as about 55ml, no more than 2-3 ml of bone marrow is generally taken at one time.Between each successive 2-3 ml draw, the needle is generally advancedinto the marrow cavity further and rotated by 90°. The reason for thisis that if too much bone marrow is drawn from one area, the sample canbe diluted with peripheral blood. In a typical surgical environment,manually advancing and rotating the needle by 90° can be a cumbersomeand time consuming process. Thus, there is a need for improved devicesand methods for aspirating bone marrow from bone, as well as aspiratingbiological components generally.

SUMMARY

The present teachings provide for an aspiration assembly including anouter cannulated needle having a tip, a threaded collar, and an inneraspiration needle. The threaded collar is mounted to an end of the outercannulated needle opposite to the tip. The inner aspiration needle has atip at a first end and a threaded portion proximate to a second end. Theinner aspiration needle is inserted within the outer cannulated needlesuch that the tip of the inner aspiration needle extends beyond the tipof the outer cannulated needle and the threaded portion of the inneraspiration needle threadably engages the threaded collar. The distancethat the tip of the inner aspiration needle extends from the tip of theouter cannulated needle increases when the inner aspiration needle isrotated in a first direction relative to the outer cannulated needle.The distance that the tip of the inner aspiration needle extends fromthe tip of the outer cannulated needle decreases when the inneraspiration needle is rotated in a second direction relative to the outercannulated needle. The second direction is opposite to the firstdirection.

The present teachings further provide for an aspiration assemblyincluding an outer cannulated needle having a needle tip and a handle, acollar having internal threads, and an inner aspiration needle. Theinner aspiration needle has a tip at a first end, a threaded portionproximate to a second end, and a needle portion between the tip of theinner aspiration needle and the second end of the inner aspirationneedle. A rigid stylet has a tip at a first end, an interlocking deviceat a second end, and a needle portion extending between the tip of thestylet and the interlocking device. The assembly includes a firstconfiguration in which the rigid stylet mates with one of the outercannulated needle or the collar such that the interlocking device of therigid stylet is attached to one of the handle or the collar. The needleportion of the stylet extends through the needle tip of the outercannulated needle. The tip of the rigid stylet extends beyond the needletip of the outer cannulated needle. The assembly includes a secondconfiguration in which the collar is mounted to the handle of the outercannulated needle. The inner aspiration needle is connected to thecollar through cooperation between the internal threads of the collarand the threaded portion of the inner aspiration needle. The needleportion of the inner aspiration needle extends through the needle tip ofthe outer cannulated needle. The tip of the inner aspiration needleextends beyond the needle tip of the outer cannulated needle.

The present teachings further provide for a method for aspirating bonemarrow from bone. The method includes the following: inserting a rigidstylet through an outer cannulated needle such that a stylet tip of therigid stylet extends beyond a needle tip of the outer cannulated needle;inserting the rigid stylet and the outer cannulated needle through abone cortex and into a bone marrow cavity; withdrawing the rigid styletfrom the bone; connecting an inner aspiration needle to a collar havinginternal threads mounted to the outer cannulated needle such that aneedle portion of the inner aspiration needle extends through the needletip of the outer cannulated needle and a threaded portion of the inneraspiration needle threadably engages the internal threads of the collar;aspirating a first quantity of bone marrow from the bone marrow cavitythrough ports in a tip portion of the inner aspiration needle; rotatingthe inner aspiration needle in a first direction relative to the outercannulated needle to move the inner aspiration needle from a firstposition in the bone marrow cavity to a second position in the bonemarrow cavity; and aspirating a second quantity of bone marrow from thebone marrow cavity through the inner aspiration needle with the inneraspiration needle in the second position, the depth of the inneraspiration needle in the bone marrow cavity when in the first positionis different from the depth of the inner aspiration needle in the bonemarrow cavity when in the second position.

The present teachings also provide for an aspiration assembly includingan outer cannulated needle, a collar, an inner aspiration needle, and astylet. The outer cannulated needle has a needle tip and a handle. Thecollar has internal threads. The inner aspiration needle has an open tipat a first end, a connection device at a second end, a threaded portionproximate to the second end, and a needle portion between the tip of theinner aspiration needle and the second end of the inner aspirationneedle. The stylet has a tip at a first end, an interlocking device at asecond end, and a needle portion extending between the tip of the styletand the interlocking device. The collar is mounted to the handle of theouter cannulated needle. The inner aspiration needle is connected to thecollar through cooperation between the internal threads of the collarand the threaded portion of the inner aspiration needle. The needleportion of the inner aspiration needle extends through the needle tip ofthe outer cannulated needle. The tip of the inner aspiration needleextends beyond the needle tip of the outer cannulated needle. The styletis connected to the inner aspiration needle through cooperation betweenthe interlocking device of the stylet and the connection device of theinner aspiration needle. The needle portion of the stylet extendsthrough the open tip of the inner aspiration needle and the tip of thestylet extends beyond the open tip of the inner aspiration needle.

The present teachings further provide for a method for aspirating bonemarrow from bone. The method includes the following: connecting a collarhaving internal threads to an outer cannulated needle; connecting aninner aspiration needle to the collar such that a needle portion of theinner aspiration needle extends through a needle tip of the outercannulated needle and a threaded portion of the inner aspiration needlethreadably engages the internal threads of the collar; connecting astylet to the inner cannulated needle such that a needle portion of thestylet extends through a needle tip of the inner cannulated needle;penetrating a bone marrow cavity of a bone using the stylet such thatthe stylet, inner aspiration needle, and the outer cannulated needle areseated within the bone; removing the stylet from cooperation with theinner cannulated needle and from within the bone; aspirating a firstquantity of bone marrow from the bone marrow cavity through the inneraspiration needle; rotating the inner aspiration needle in a firstdirection relative to the outer cannulated needle to move the inneraspiration needle from a first position in the bone marrow cavity to asecond position in the bone marrow cavity; and aspirating a secondquantity of bone marrow from the bone marrow cavity through the inneraspiration needle with the inner aspiration needle in the secondposition, the depth of the inner aspiration needle in the bone marrowcavity when in the first position is different from the depth of theinner aspiration needle in the bone marrow cavity when in the secondposition.

Further areas of applicability will become apparent from the descriptionprovided herein. It should be understood that the description andspecific examples are intended for purposes of illustration only and arenot intended to limit the scope of the present disclosure.

DRAWINGS

The drawings described herein are for illustration purposes only and arenot intended to limit the scope of the present disclosure in any way.

FIG. 1A is a side view of an aspiration needle assembly according to thepresent teachings;

FIG. 1B is a cross-sectional view of the aspiration needle assembly ofFIG. 1A taken along line 1B;

FIG. 2A is a side view of an outer aspiration needle of the needleassembly of FIGS. 1A and 1B;

FIG. 2B is an end view of the outer aspiration needle of FIG. 2A;

FIG. 3A is a side view of an inner aspiration needle of the needleassembly of FIGS. 1A and 1B;

FIG. 3B is a cross-sectional view of the inner aspiration needle of FIG.3A taken along line 3B;

FIG. 3C is a close-up view of area 3C of FIG. 3A;

FIG. 4A is a side view of a threaded collar of the aspiration needleassembly of FIGS. 1A and 1B;

FIG. 4B is a cross-sectional view of the threaded collar of FIG. 4Ataken along line 4B of FIG. 4A;

FIG. 4C is a close-up view of area 4C of FIG. 4B;

FIG. 5A is a perspective view of a stylet according to the presentteachings for use with the needle assembly of FIGS. 1A and 1B;

FIG. 5B is an end view of the stylet of FIG. 5A;

FIG. 6 is a cross-sectional view of the stylet in cooperation with theouter aspiration needle;

FIG. 7A is a cross-sectional view of the aspiration needle assembly withthe inner aspiration needle in a first position in which the inneraspiration needle extends a first length L1 beyond the tip of the outerneedle;

FIG. 7B is a cross-sectional view of the aspiration needle assembly withthe inner aspiration needle in a second position in which the inneraspiration needle extends a second length L2 beyond the tip of the outerneedle;

FIG. 8A is a cross-sectional view of an additional aspiration assemblyaccording to the present teachings;

FIG. 8B is a close-up view of area 8B of FIG. 8A; and

FIG. 8C is a close-up view of a tip portion of the aspiration assemblyof FIG. 8A.

DETAILED DESCRIPTION

The following description is merely exemplary in nature and is notintended to limit the present disclosure, application, or uses.

With initial reference to FIGS. 1A and 1B, an aspiration needle assemblyaccording to the present teachings is illustrated at reference numeral10. The aspiration needle assembly 10 generally includes an outer needle12, an inner aspiration needle 14, and a threaded collar 16.

With additional reference to FIGS. 2A and 2B, the outer needle 12generally includes a handle 18, a female Luer lock 20, and a needleportion 22. The Luer lock 20 extends from a first end of the handle 18and the needle portion 22 extends from a second end of the handle 18that is opposite to the first end. The Luer lock 20 is recessed withinthe handle 18. The handle 18 includes winged portions 19 a and 19 b thatare on opposite sides of the Luer lock 20. The Luer lock 20 can be anysuitable type of device for fastening two components together. The Luerlock 20 includes a sidewall 21 having a first diameter portion 21 aproximate to the handle 18 and a second diameter portion 21 b that isdistal to the handle 18. The second diameter portion 21 b has externalthreads and has a diameter that is greater than the first diameterportion 21 a. The needle portion 22 is cannulated and is in fluidcommunication with the female Luer lock 20 to permit passage ofmaterials through the outer needle 12. The needle portion 22 has apointed open tip 24. The pointed tip 24 can have any suitable shape,such as a conical tip having a sharpened end, as illustrated.

With additional reference to FIGS. 3A-3C, the inner aspiration needle 14generally includes a female Luer lock 26, a handle portion 27, athreaded portion 28, and a needle portion 30 having a tip 32.

The female Luer lock 26 is at an end of the inner aspiration needle 14that is opposite to the tip 32. The female Luer lock 26 can be anysuitable fastening device for connecting the inner aspiration needle 14to a syringe or other suitable device for withdrawing material throughthe inner aspiration needle 14. As illustrated, the female Luer lock 26includes external threads 29.

The threaded portion 28 of the inner aspiration needle 14 is proximateto the female Luer lock 26. The threaded portion 28 is substantiallycylindrical and includes an externally threaded sidewall. The tip 32 isconical and has a closed end. The tip 32 includes a plurality of ports34 around its outer diameter to permit the passage of material into theinner aspiration needle 14. The ports 34 are spaced apart around theouter diameter of the tip 32 to permit intake of materials from anentire area encompassing the tip 32. The inner aspiration needle 14 iscannulated and includes a through bore 33 that provides forcommunication of material from the ports 34 through the inner aspirationneedle 14 to the female Luer lock 26.

With additional reference to FIGS. 4A, 4B, and 4C, the threaded collar16 defines an inner housing 36 and a male Luer lock 38. The innerhousing 36 includes an inner threaded sidewall 40 in which the threadsare sized and shaped to cooperate with the threaded portion 28 of theinner aspiration needle 14 to form an aspiration needle assembly 10 inwhich the distance that the tip 32 of the aspiration needle 14 extendsfrom the tip 24 of the needle portion 22 of the handle 12 can be meteredby rotation of the inner aspiration needle 14 within the threaded collar16. The male Luer lock 38 includes a conical extending portion 42 and anouter skirt 43 that defines a recessed portion 44 therebetween. Therecessed portion 44 receives the Luer lock 20 and includes threads 45that cooperate with the threaded portion 21 b of the female Luer lock 20to enhance the connection between the male Luer lock 38 and the femaleLuer lock 20. A through port 46 extends through the extending portion 42to the inner housing 36. One skilled in the art will recognize that thethreaded collar 16 can be integral with the Luer lock 20 of the handle12 or can be a separate part, as illustrated in FIGS. 4A, 4B, and 4C,which can securely mate with the Luer lock 20.

With additional reference to FIGS. 5A and 5B, a stylet or trocar isillustrated at reference numeral 48. The stylet 48 generally includes aneedle portion 50 and an end cap 52. The needle portion 50 is rigid andsolid and includes a pointed end 54. The end cap 52 is mounted to thestylet 48 at an end opposite to the pointed end 54. The end cap 52includes a male Luer lock with threads 56 to permit the stylet 48 to beconnected to the threaded portion 21 b of the female Luer lock 20 of theouter needle 12, as illustrated in FIG. 6.

Use of the assembly 10 and the stylet 48 will now be described. Theassembly 10 and the stylet 48 can be used for a variety of differentpurposes. For example and as described herein, the assembly 10 and thestylet 48 can be used to extract bone marrow from bone.

As illustrated in FIG. 6, the stylet 48 is mated with the outer needle12 such that the needle portion 50 of the stylet 48 extends through thehandle 18 and through the open tip 24 of the outer needle 12. The Luerlock threads 56 of the end cap 52 cooperate with the threaded portion 21b of the female Luer lock 20 of the handle 18 to secure the stylet 48 tothe outer needle 12. When the threaded collar 16 is integral with theouter needle 12, the stylet 48 is inserted through the threaded collar16 and provided with an extended length so that the pointed end 54extends from the tip portion 24 of the outer needle 12. Further, the endcap 52 can be configured to mate with the end of the threaded collar 16that is opposite to the Luer lock 38.

With the stylet 48 secured to the outer needle 12, the stylet 48 and theouter needle 12 are advanced into the bone cortex. The pointed end 54 ofthe stylet 48 protrudes out beyond the needle portion 22 of the outerneedle 12 to make initial contact with the bone. The pointed end 54 hasa sharp surface to facilitate insertion of the stylet 48 and the outerneedle 12 into the bone. Thus, the rigid stylet needle portion 50facilitates penetration of the bone and prevents bone and othermaterials from entering and clogging the tip portion 24 of the outerneedle 12. The stylet 48 and the handle 12 are advanced into the bonemarrow cavity to a point where bone marrow can be aspirated.

The stylet 48 is removed and the threaded collar 16 is attached to thehandle 18. Specifically, the male Luer lock 38 of the threaded collar 16is attached to the female Luer lock 20 of the handle 18 by inserting theouter cylindrical sidewall 21 of the female Luer lock 20 within therecessed portion 44 of the male Luer lock 38 so that the threads 45engage the threaded second diameter portion 21 b of the sidewall 21. Theaspiration needle 14 is inserted through the threaded collar 16 and theouter needle 12 such that the tip portion 32 extends out through andbeyond the tip portion 24 and the threaded portion 28 threadably mateswith the threads 40 of the threaded collar 16. Positioning the inneraspiration needle 14, as illustrated in FIG. 7A, allows the tip 32 ofthe inner aspiration needle 14 to protrude from the outer needle 12 to afirst length L1, which is far enough from the needle portion 22 of theouter needle 12 to aspirate the first two to three milliliters of bonemarrow.

Bone marrow is aspirated by attaching a suitable device, such as asyringe, to the female Luer lock 26 of the inner aspiration needle 14.The presence of the ports 34 at the tip 32 facilitates aspiration ofbone marrow through the inner aspiration needle 14 from the areasurrounding the tip 32.

To aspirate additional bone marrow, the inner aspiration needle 14 isthreaded further into the threaded collar 16 a predetermined number ofdegrees, such as about 90°, so that the inner aspiration needle 14protrudes from the outer needle 12 to a second length L2 (FIG. 7B),which is greater than the first length L1. Rotating the inner aspirationneedle 14 in this manner, while keeping the outer needle 12 stationary,advances the tip 32 of the needle 30 deeper into the bone and rotatesthe tip 32 such that the ports 34 are perpendicular to their previousposition. Thus, the outer needle 12 serves as a guide for the inneraspiration needle 14 to direct the inner aspiration needle 14 to thebone. This process is performed as many times as needed to obtain adesired amount of bone marrow.

Withdrawing bone marrow using the assembly 10 provides numerousadvantages. For example, rotating the inner aspiration needle 14 so thatits position in the bone changes permits withdrawal of bone marrow fromdifferent areas of the bone to prevent the aspirated marrow frompossibly being diluted with peripheral blood.

As an alternative to advancing the inner aspiration needle 14 into thebone as additional bone marrow is withdrawn, bone marrow aspiration canbegin with the threaded portion 28 of the inner aspiration needle 14 infull threaded engagement with the threaded collar 16. As additional bonemarrow is aspirated, the inner aspiration needle 14 can be rotated, forexample counterclockwise at 90°, to withdraw the inner aspiration needle14 out from within the outer needle 12.

Further, any suitable metering device that provides control over thedistance that the inner aspiration needle 14 extends from the outerneedle 12 may be used in place of the threaded collar 16. For example, acollar having a series of spaced apart notches for cooperating with tabsspaced apart on the inner aspiration needle 14 can be used. Further, inplace of the threaded portion 28 and the threaded sidewall 40 can beopposing surfaces with any suitable friction generating surface so as toprovide control over the distance that the inner aspiration needle 14extends from the outer needle 12.

An additional bone marrow aspiration needle assembly is illustrated inFIGS. 8A, 8B, and 8C at reference numeral 110. The assembly 110 issimilar to the assembly 10. As such, similar features are designatedusing like reference numbers, but increased by 100. With respect to suchsimilar features, the above description of the assembly 10 also appliesto the assembly 110.

As with the assembly 10, the assembly 110 generally includes an outerneedle 112, an inner needle 114, a threaded collar 116, and a stylet148. However, unlike the inner needle 14 of the assembly 10, the innerneedle 114 of the assembly 110 is cannulated across its entire length.The cannulated inner needle 114 includes a handle portion 127, a Luerlock connector 126, a threaded portion 128, a needle portion 130, anopen tip 132, and a plurality of ports 134 (FIG. 8C) around its outerdiameter proximate to the open tip 132.

The assembly 110 further includes a stylet 148. The stylet 148 generallyincludes a rigid needle portion 150 having an end cap 152 at one end anda needle tip 154 at an opposite end. The end cap 152 includes a Luerlock having threads 156 for connecting the stylet 148 to externalthreads 129 of the Luer lock 126 of the inner needle 114, as illustratedin FIGS. 8A and 8B. The needle portion 150 of the stylet 148 is of asufficient length such that the tip 154 extends beyond the tip 132 ofthe inner needle 114 when the stylet 148 is mounted to the inner needle114.

With the stylet 148 mounted to the inner needle 114 and extending therethrough, the assembly 110 can be inserted directly into the bone cortexin the same manner described above with respect to the assembly 10, adifference being that the inner needle 114 is inserted together with thestylet 148 and the outer needle 112. Because the tip 154 of the stylet148 extends beyond the tip 132 of the inner needle 114, it is the stylet148 that first penetrates the bone cortex.

After the assembly 110 is inserted into the bone cortex, the stylet 148is removed from cooperation with the inner needle 114 and a suitabledevice, such as a syringe, is attached to the Luer lock 126 of the innerneedle 114 to aspirate bone marrow. The assembly 110 is then used toaspirate bone marrow in a similar manner as described above with respectto the use of the assembly 10. Thus, with the assembly 110 thecannulated inner needle 114 and the threaded collar 116 can both bemounted to the outer needle 112 prior to insertion of the assembly 110into the bone cortex.

The description of the invention is merely exemplary in nature and,thus, variations that do not depart from the gist of the invention areintended to be within the scope of the invention. Such variations arenot to be regarded as a departure from the spirit and scope of theinvention.

1. A method for aspirating bone marrow from bone comprising: inserting arigid stylet through an outer cannulated needle such that a stylet tipof the rigid stylet extends beyond a needle tip of the outer cannulatedneedle; inserting the rigid stylet and the outer cannulated needlethrough a bone cortex and into a bone marrow cavity; withdrawing therigid stylet from the bone; connecting an inner aspiration needle to acollar having internal threads mounted to the outer cannulated needlesuch that a needle portion of the inner aspiration needle extendsthrough the needle tip of the outer cannulated needle and a threadedportion of the inner aspiration needle threadably engages the internalthreads of the collar; aspirating a first quantity of bone marrow fromthe bone marrow cavity through ports in a tip portion of the inneraspiration needle; rotating the inner aspiration needle in a firstdirection relative to the outer cannulated needle to move the inneraspiration needle from a first position in the bone marrow cavity to asecond position in the bone marrow cavity; and aspirating a secondquantity of bone marrow from the bone marrow cavity through the inneraspiration needle with the inner aspiration needle in the secondposition, the depth of the inner aspiration needle in the bone marrowcavity when in the first position is different from the depth of theinner aspiration needle in the bone marrow cavity when in the secondposition.
 2. The method of claim 1, wherein the ports of the inneraspiration needle rotate about 90° when the inner aspiration needle ismoved from the first position to the second position.
 3. The method ofclaim 1, wherein aspirating the first quantity includes aspirating about2-3 ml of bone marrow; and wherein aspirating the second quantityincludes aspirating about 2-3 ml of bone marrow.
 4. The method of claim1, wherein the second position of the inner aspiration needle is deeperwithin the bone marrow cavity than the first position of the inneraspiration needle.
 5. The method of claim 1, wherein the first positionof the inner aspiration needle is deeper within the bone marrow cavitythan the second position of the inner aspiration needle.
 6. The methodof claim 1, further comprising attaching a syringe to the inneraspiration needle with a Luer lock to aspirate the bone marrow throughthe inner aspiration needle.
 7. The method of claim 1, furthercomprising mating the collar to the outer handle with a Luer lock. 8.The method of claim 1, wherein rotating the inner aspiration needle fromthe first position to the second position includes rotating the inneraspiration needle about 90°.
 9. A method for aspirating bone marrow frombone comprising: connecting a collar having internal threads to an outercannulated needle; connecting an inner aspiration needle to the collarsuch that a needle portion of the inner aspiration needle extendsthrough a needle tip of the outer cannulated needle and a threadedportion of the inner aspiration needle threadably engages the internalthreads of the collar; connecting a stylet to the inner cannulatedneedle such that a needle portion of the stylet extends through a needletip of the inner cannulated needle; penetrating a bone marrow cavity ofa bone using the stylet such that the stylet, the inner aspirationneedle, and the outer cannulated needle are seated within the bone;removing the stylet from cooperation with the inner cannulated needleand from within the bone; aspirating a first quantity of bone marrowfrom the bone marrow cavity through the inner aspiration needle;rotating the inner aspiration needle in a first direction relative tothe outer cannulated needle to move the inner aspiration needle from afirst position in the bone marrow cavity to a second position in thebone marrow cavity; and aspirating a second quantity of bone marrow fromthe bone marrow cavity through the inner aspiration needle with theinner aspiration needle in the second position, the depth of the inneraspiration needle in the bone marrow cavity when in the first positionis different from the depth of the inner aspiration needle in the bonemarrow cavity when in the second position.
 10. The method of claim 9,wherein aspirating the first quantity includes aspirating about 2-3 mlof bone marrow; and wherein aspirating the second quantity includesaspirating about 2-3 ml of bone marrow.
 11. The method of claim 9,wherein the second position of the inner aspiration needle is deeperwithin the bone marrow cavity than the first position of the inneraspiration needle.
 12. The method of claim 9, wherein the first positionof the inner aspiration needle is deeper within the bone marrow cavitythan the second position of the inner aspiration needle.
 13. The methodof claim 9, further comprising attaching a syringe to the inneraspiration needle with a Luer lock to aspirate the bone marrow throughthe inner aspiration needle.
 14. The method of claim 9, wherein rotatingthe inner aspiration needle from the first position to the secondposition includes rotating the inner aspiration needle about 90°.
 15. Amethod for aspirating bone marrow from bone comprising: providing anouter cannulated needle having a first tip at a first end and a firstcoupling device at a second end that is opposite to the first end;providing a threaded collar having a second coupling device incooperation with the first coupling device to removably couple thethreaded collar to the outer cannulated needle; and providing an inneraspiration needle having a second tip at a third end, a third couplingdevice at a fourth end opposite to the third end, and a threaded portionproximate to the fourth end; inserting the inner aspiration needlewithin the outer cannulated needle such that the second tip of the inneraspiration needle extends beyond the first tip of the outer cannulatedneedle and the threaded portion of the inner aspiration needlethreadably engages the threaded collar; increasing a distance that thesecond tip of the inner aspiration needle extends from the first tip ofthe outer cannulated needle by rotating the inner aspiration needle in afirst direction relative to the outer cannulated needle; and decreasingthe distance that the second tip of the inner aspiration needle extendsfrom the first tip of the outer cannulated needle by rotating the inneraspiration needle in a second direction relative to the outer cannulatedneedle, wherein the second direction is opposite to the first direction.16. The method of claim 15, further comprising: connecting a stylet tothe inner cannulated needle such that a needle portion of the styletextends through a needle tip of the inner cannulated needle; penetratinga bone marrow cavity of a bone using the stylet such that the stylet,the inner aspiration needle, and the outer cannulated needle are seatedwithin the bone; and aspirating a first quantity of bone marrow from thebone marrow cavity through the inner aspiration needle.
 17. The methodof claim 16, further comprising: removing the stylet from cooperationwith the inner cannulated needle and from within the bone prior toaspirating the first quantity of bone marrow.
 18. The method of claim17, further comprising: rotating the inner aspiration needle in thefirst direction relative to the outer cannulated needle to move theinner aspiration needle from a first position in the bone marrow cavityto a second position in the bone marrow cavity; and aspirating a secondquantity of bone marrow from the bone marrow cavity through the inneraspiration needle with the inner aspiration needle in the secondposition, the depth of the inner aspiration needle in the bone marrowcavity when in the first position is different from the depth of theinner aspiration needle in the bone marrow cavity when in the secondposition.